Universal lentiviral vector luciferase reporter backbone for tracking expression of nearly any miRNA
Currently available:
Lv-miR-control vector (lacks a miRNA target sequence)
Lv-Luc-miR-145-5p
Lv-Luc-miR-27b-5p
Lv-Luc-miR-27b-3p
NEW! Lv-Luc-miR-21-3p (validation in process)
Next generation RNA-sequencing (RNA-seq)
Available Reporter Vectors (validated)
NEW! Human Aggrecan promoter reporter (Lentiviral vector, dTomato)
Human Aggrecan promoter reporter (Lentiviral vector, secreted Gaussia Luciferase)
Human COL10A1 promoter reporter (Lentiviral vector, secreted Gaussia Luciferase)
Human COL2A1 promoter reporter (Lentiviral vector, secreted Gaussia Luciferase)
Human Col2 promoter reporter (Plasmid, Firefly Luciferase)
Human Osteocalcin promoter reporter (Lentiviral vector, Firefly Luciferase)
NEW! Human Osteocalcin promoter reporter (Lentiviral vector, dTomato)
Human Runx2 promoter reporter (Lentiviral vector, secreted Gaussia Luciferase)
Human Sox9 promoter reporter (Lentiviral vector, Firefly Luciferase)
Human Sox9 promoter reporter (Lentiviral vector, secreted Gaussia Luciferase)
Human Sox9 promoter reporter short sequence (Plasmid, Firefly Luciferase)
Human Sox9 promoter reporter full sequence (Plasmid, Firefly Luciferase)
Mouse Sox9 promoter reporter full sequence (Plasmid, Firefly Luciferase)
Human Sox9 promoter reporter (Lentiviral vector, eGFP)
Available lentiviral vectors for cell tracking:
pLV-Puromicin-dTomato-Luciferase
pLV-Puromicin-CyPet
pLV-Puromicin-YPet
pLV-Puromicin-Luciferase
pLV-Blasticidin-mCherry-Luciferase
pLV-Neomicin-dTomato
pLV-Puromicin-eGFP
NEW! pLV-Secreted Nano-Luciferase
NEW! pLV-Puromicin-eGFP-Luciferase
Generation of differentiation markers / promoter-specific viral vectors with traceable gene reporters.
Efficient viral transduction of primary cells and cell lines.
Large-scale generation of hybrid structures for in vitro chondrogenesis.
Bioluminescence Imaging (BLI) of created hybrid structures to assess cell differentiation in real time.
Structural analysis of engineered tissue sections (Immunohistochemistry) with a panel of Proteoglycan (PG)- and Glycosaminoglycan (GAG)-specific antibodies.
Immunological assessment of conditioned medium (ELISA) with the same panel of specific antibodies, as an indirect analysis of ECM remodeling.
Biochemical determination of the GAG composition of generated cartilage ECM.
Biochemical environment services
Transport modeling and parameter estimation for glucose consumption in aggregate culture
NEW! Biomolecular rate cut-off determination for success/failure in aggregate culture.
Donor-specific assessment of metabolism of client-MSC aggregates (glucose, lactate)
Donor-specific assessment of metabolism of client-MSC aggregates under varying exogenous factors (to be specified by clients)
NEW! LC/QQQ Amino acid analysis of culture samples
NEW! Amino acid pathway flux balance analysis
Microbioreactor and splitter for feeding for use in MSC chondrogenesis
